Global Council for Innovation in Rapeseed and Canola

12th IRC, Wuhan, China, March 26-30, 2007 -  Volume 2

Sustainable Development in Cruciferous Oilseed Crops Production

BIOTECHNOLOGY

Genomics and Its Applications

Korea Brassica genome resource bank (KBGRB): integrated genomic resources of Brassica species 
Y. H. Jin, C. P. Hong, S. R. Choi,G. S. Sin, Y. P. Lim 2
Alpha-tocopherol value added Brassica juncea: fortified human nutrition and abiotic stress tolerance to the plant
Mohd. Aslam Yusuf,Prem Kumar Gupta, Neera Bhalla Sarin 5
Brassica napus plant regeneration and transformation via agrobacterium-mediated-transformation
Alireza Zebarjadi, Mokhtar Jalali Javaran, Ali Hatef Salmanian, Ghasem Karimzadeh 9
Progress in producing a genetic transformation sytem for salt tolerant rapeseed in Bangladesh
Lutful Hassan, S.K. Talukder 12
High-frequency non-selective transformation method in Brassica napus for obtaining marker-free plants
WANG Jingqiao, CHEN Wei, LI Jingfong, ZHANG Guojian, DONG Yunsong, LI Genze 15
In vitro selection of microspore derived embryo genotypes based on molecular marker and oil quality analysis in oilseed rape (Brassica napus L.)
Ujjal Kumar Nath, Mohammed C. Iqbal, Christian Möllers 20
Transformation of microspore-derived embryos of winter oilseed rape (Brassica napus L.) using Agrobacterium tumefaciens 
Teresa Cegielska-Taras, Tomasz Pniewski, Laurencja SzaB
a 24
Molecular characterisation of microspore-derived progeny from the interspecific F1 of Brassica napus × B. carinata
Matthew N. Nelson,Annaliese Mason, Marie-Claire Castello, Clare E. O'Lone, Guijun Yan, Sheng Chen, Wallace A. Cowling 27
Genetic mapping of clubroot resistance genes in oilseed rape
Susanne Werner, Elke Diederichsen, Martin Frauen, Jörg Schondelmaier,Christian Jung 30
‘The latest in seed coat fashion’: seed colour (proanthocyanidin) and trichome mutations in a new population of activation-tagged Arabidopsis lines
Margaret Gruber, Dejun Cui, Limin Wu, Bruce Coulman, Isobel Parkin 34
Gene cloning and transgenic development in Indian mustard (Brassica juncea L. Czern) for improved tolerance to abiotic stresses
K.C. Bansal, Deepti Tayal, Monica Dalal, V. Chinnusamy, AK Singh, Shiv Shanker 38
An efficient Agrobacterium – mediated transformation method used in Chinese cabbage (Brassica rapa ssp. pekinensis )
Zhi Hong Yang,Md. Jamil Hossain, Seo Young Song , Hyo Yeon Lee, Han Dae Yoon, Yong Pyo Lim 42
Transformation of Brassica napus with the method of floral-dip
FU Shaohong, WEI Xianya, NIU Yingze, GUO Shixing 45
The lignin biosynthesis regulation and its relationship with Sclerotinia and lodging resistances for Brassica napus
YANG Xiangdong, WANG Hanzhong, LIU Guihua, WANG Xinfa 50
Mutagenic treatments of cotyledons for in vitro plant regeneration in oilseed rape
HE Yun, WAN Guanglong, JIN Zonglai, XU Ling, TANG Guixiang, ZHOU Weijun 54
Improvement of erucic acid level in Brassica napus L. 
Yudal Yoon Hi, Choy, Yu Jeong, Jeong, Min Jung, Kim, Hyun Mi, Park, June Seung,Lee, Young Suk, Jang, In Sun, Han, Moon Sun, Yoon, Young Ho, Yoon 58
The effects of different selective agents on seed germinating and callus forming in different cultivars in Brassica napus 
ZHANG Lili,TAN Xiaoli, KONG Fanming 62
Studied on artificial resynthesis of B . napus from B. olerecea var. aceaphala and B. camptris through embryo rescue
ZHOU Qingyuan,LI Jiana, YIN jiaming, CUI Cui, TANG Zhanglin, CHEN Li, LI Na 65
Effects of mutagenic treatments of isolated microspores and microspore-derived embryos on embryogenesis and plant regeneration in oilseed rape
HE Yun, WAN Guanglong, CHEN Shaobao, JIN Zonglai, XU Ling, TANG Guixiang, ZHOU Weijun 69
Development, cytological and molecular analysis and breeding potential of amphidiploids between Raphanus Sativus and Brassica Alboglabra
CHEN Honggao, WU Jiangsheng, LIU Chao, WANG Fang, CHENG Xiaomao, LI Yuan 73
Genetic variability between several Brasicaceae populations of different winter survival ability
Branislava Raki
, Ana Marjanovi
-Jeromela,Radovan Marinkovi
, Dejana Safti
-Pankovi
76
Studies on gene flow from GM herbicide-tolerant rapeseed (B. napus) to other species of crucifers
PU Huiming, QI Cunkou,ZHANG Jiefu, FU Shouzhong, GAO Jianqin, CHEN Xinjun, CHEN Song 79
Is returning to non-GM rapeseed varieties doomed to fail?
Antoine Messéan, Christophe Sausse, Jacques Gasquez, Henri Darmency 82
The establishment of high frequency somatic embryogenesis and plant regeneration system of Eruca sativa 
MillZHANG Tao 85
Comparsions of developmental stages of microspore by bud size and embryogenesis from its microspore in Brassica species
Young Seok Jang, Cheul Woo Kim, In Hoo Choi, Jin Ki Bang 88
Analysis of glucosinolate components of different DH populations in Brassica napus LEI Weixia, FAN Zhixiong, SUN Xiuli, YANG Liyong,HE Dongli, YANG Guangsheng, ZHAO Jianyi 92
Gene flow from transgenic oilseed rape to Chinese conspecific landrace under field conditions and its implication for China 
WEI Wei ,SHEN Baocheng, TANG Zhixi, MI Xiangcheng 96
Cloning of cDNA encoding F5H from Brassica napus and regulation of lignin biosynthesis by expressing antisense gene in transgenic tobacco
SHI Zhujuan, LIU Guihua, SHEN Jinxiong, WANG Hanzhong, YANG Xiangdong 99
Towards low sinapine oilseed rape
Juliane Mittasch, Alfred Baumert, Dieter Strack, Carsten Milkowski 102
QTL analysis of flowering time and photoperiod sensitivity in rapeseed (Brassica napus L.)
CAI Changchun, TU Jinxing, FU Tingdong,CHEN Baoyuan 106
Selection for low seed dormancy in GM oilseed rape
Sabine Gruber, Wilhelm Claupein 112

BIOTECHNOLOGY
Gene Cloning and Functional Analysis

Identification of fad2 mutations and development of Allele-Specific Markers for High Oleic acid content in rapeseed (Brassica napus L.)
Falentin Cyril, Brégeon Michel, Lucas Marie-Odile, Deschamps Max, Leprince Françoise, Fournier Marie-Thérèse,Delourme Régine, Renard Michel 117
Serial Analysis of Gene Expression (SAGE) applied to the study of Brassica napus seed development
Christian Obermeier, Bashir Hosseini, Rod Snowdon 120
Characterization of genes of importance in stress resistance in mustard, Brassica juncea
Sravan Kumar Jami, T. Swathi Anuradha, G. Meur,Aparna Datta-Gupta, S. Prakash, P.B. Kirti* 124
Molecular cloning of two genes encoding cinnamate 4-hydroxylase (C4H) from oilseed rape (Brassica napus)
CHEN Anhe,CHAI Yourong, LI Jiana, CHEN Li 127
Manipulation of biosynthesis of aliphatic glucosinolates by gene replacement and RNAi gene silencing
Zhixia Niu, Muqiang Gao,Peter B. E. McVetty, Fouad Daayf, Hongyan Zhu, Carlos F. Quiros, Genyi Li 131
An EMS population of Brassica napus L. for tilling
Felix Dreyer , Martin Frauen, Gunhild Leckband, Carsten Milkowski,Christian Jung 134
Analysis of a set of 14,549 Brassica napus unique ESTs and signature genes related to Sclerotinia resistance
MAO Han,DONG Caihua, SU Zhen, JI Ruiqin, GUO Xuelan, LIU Shengyi 138
Isolation and analysis of differentially expressed genes in dominant genic male sterility (DGMS) Brassica napus L.
WU Jianyong,LIU Pingwu, HONG Dengfeng, YANG Guangsheng 141
Inheritance and molecular mechanism of the genic male sterility accession “Shaan-GMS ” in Brassica napus L.
HU Shengwu, YU Chengyu,ZHAO Huixian, LIU Shengyi, WANG Hanzhong 149
Genetic engineering and breeding of Long-Chain Polyunsaturated Fatty Acids (LCPUFA) in Rapeseed
Amine Abbadi, Martin Fulda,Ivo Feussner, José Orsini, Jens Weyen, Martin Frauen, Gunhild Leckband 152
A novel mutation for low erucic acid found in Brassica napus cv. Zhongshuang No.9: gene cloning and molecular characterization
LU Changming, WU Gang, WU Yuhua, XIAO Ling, LI Xiaodan,WANG Hanzhong 156
Terminal-Repeat Retrotransposon In Miniature (TRIM) elements and their potential utility as DNA markers in Brassica relatives
Tae-Jin Yang, Soo-Jin Kwon, Jung Sun Kim, Mina Jin, Beom-Soon Choi, Yong Pyo Lim, Beom-Seok Park 159
Generation of recombinant antibodies specific for Sclerotinia sclerotiorum
LIAO Yucai , LI Heping , LIU Wei , XINg Shu , LIU Jinlong ,ZHANG Jingbo 162
Molecular cloning and expression analyses of a putative triacylglycerol lipase gene from Brassica napus
ZHOU Jia, TAN Xiaoli,XU Tao 166
Molecular cloning of two ortholog genes of Arabidopsis thaliana TTG1 from oilseed rape (Brassica napus L.)
LU Jun, LI Jiana,WANG Sangen, LEI Bo, CHAI Yourong 170
Molecular cloning and functional identification of BnIND gene in Brassica napus 
XIA Zongwei, TAN Xiaoli, ZHANG Zhiyan 173
Transcriptional control of mitochondrial genes in different tissues of NCa CMS system in Brassica napus L. and their relationship with sterility
WEI Wenliang, WANG Hanzhong, LIU Guihua 178
Identification of the corresponding avirulence gene of Leptosphaeria maculans to the resistance gene (LepR1) in Brassica napus.
Kaveh Ghanbarnia, Roger Rimmer, W. G. Dilantha Fernando 182
DNA damage and hypermethylation of rapeseed plants in response to salt stress
LU Guangyuan, WU Xiaoming, CHEN Biyun,GAO Guizhen, XU Kun 185
Construction of plant high-frequency RNAi vector containing inverse-repeat terminator
CHEN Wei, LI Jingfong, DONG Yunshong,LIU Yulan, ZHANG Meihua, LI Genze, WANG Jingqiao 188
An efficient method for generating cDNAs in Brassica carinata
A. Br.Supriya, Neelam R. Yadav, Ram C. Yadav, Dhiraj Singh 192
Molecular cloning and characterrization of key genes related to lipid biosynthesis from Descurainia sophia
TANG Sanyuan, GUAN Rongzhan, HUANG Ji, ZHANG Hongsheng 195
Isolation, characterization and expression of a gene from Brassica napus encoding a LIM-domain protein
HUANG Ruizhi ,WU Xuelong ; JIANG Caizhong, HU Zhanghua, HE Haiyan, CHEN Jinqing 199
Molecular cloning of Brassica napus TRANSPARENT TESTA 2 (BnTT2) gene family encoding potential MYB regulatory proteins of proanthocyanidin biosynthesis
WEI Yunliang, LI Jiana, LU Jun, TANG Zhanglin, PU Dongchun, CHAI Yourong 203
Molecular cloning, characterization and expression of two orthologs of Arabidopsis thaliana phenylalanine ammonia-lyase 1 (AtPAL1) from rapeseed (Brassica napus L.)
NI Yu, JIANG Honglin, LI Jiana, CHAI Yourong 207
Studies on transferring ”6 – fatty acid desaturase gene(D6D) into Brassica napus L. plants
LIN Na, YIN Jiaming, ZHOU Qingyuan,CHAI Yourong, LU He, LI Jiana 210
Studies on transforming Brassica napus L. with proteinase inhibitor gene DsTI1 from Descurainia sophia
DU Wenmimng, HUANG Ji, ZHANG Hongsheng, GUAN Rongzhan, DONG Haibin, TANG Sanyuan 214
Substitution of Ala-183 to Thr in 5-enolpyruvylshikimste 3-phoshpate synthase of E. coli (k12) and Transformation of Rapeseed (Brassica napus) with altered gene to tolerance of plant to Glyphosate
Danial Kahrizi, Ali Hatef Salmanian, Amir Mousavi 218
Fine mapping and cloning of the LepR3 blackleg (Leptosphaeria maculans) resistance gene in ‘Surpass 400’
 Zining Wang, Zudong Sun,Genyi Li, Peter B.E. McVetty 226
Fine mapping and physical mapping of a seed coat color gene in Chinese cabbage (Brassica rapa L.)
Jiefu Zhang, Yuxiang Yuan, Xiaowei Zhang, Jianfeng Geng, Peter B. E. McVetty, Sylvie Cloutier, Genyi Li 229
QTL mapping and epistasis analysis for plant height and height to the first branch in rapeseed (Brassica napus L.)
ZHANG Shufen, FU Tingdong, ZHU Jiacheng, WANG Jianping, WEN Yancheng, MA Chaozhi, JIANG Yuzhong 232

BIOTECHNOLOGY
Tissue Culture and Molecular Breeding

Molecular breeding of oilseed rape (Brassica napus) with modified oil quality for nutritional and non-food purposes
Wolfgang Friedt, Christof Stoll, Mirela Raclaru, M. Karim Zarhloul, Wilfried Lühs 237
Dissection of quantitative traits in oilseed rape for marker development using Brassica-Arabidopsis comparative genomics
Rod Snowdon, Maen Hasan, Niclas Freitag, Florin Lipsa, Benjamin Wittkop, Anna Stein, Ana Gloria Badani, Wolfgang Friedt 241
Fine genetic mapping and physical delimitation of the recessive genic male sterile gene Bnms1 to a 21-kb DNA segment in Brassica napus L.
YI Bin, CHEN Yuning, LEI Shaolin, MA Chaozhi, TU Jinxing, FU Tingdong 245
Genetic diversity and distinctiveness revealed by SSR markers among rapeseed (Brassica napus L.) genotypes from Australia, China and India
Sheng Chen, Matthew N. Nelson, Kioumars Ghamkhar, Wallace A. Cowling 248
Oil content related candidate gene mapping in oilseed rape
ZHAO Jianyi, NI Xiyuan, HUANG Ruizhi, XU Fei, LEI Weixia 253
Analysis of linkage disequilibrium in canola-quality winter rapeseed (Brassica napus L.)
Wolfgang Ecke, Rosemarie Clemens,Heiko C. Becker 257
Molecular and phenotypic characterization of near isogenic lines at QTL for quantitative resistance to Leptosphaeria maculans in oilseed rape (Brassica napus L.)
Delourme Régine, Piel Nathalie, Horvais Raymonde, Pouilly Nicolas, Domin Claude, Vallée Patrick, Falentin Cyril, Renard Michel 261
Introgression of Brassica rapa subsp. sylvestris blackleg resistance into B. napus
Fengqun Yu, Derek J. Lydiate, Sarah Kuzmicz,S. Roger Rimmer 264
Genetic and physical mapping of CRb, a gene conferring resistance to clubroot in Chinese cabbage
Zhongyun Piao, Young Ki Lee,Young Mi Lee, Taesik Uhm, Hong Gi Kim, and Yong Pyo Lim 269
Collinearity between AFLP markers flanking dominant genic male sterility gene in Brassica napus and homologous loci in Arabidopsis thaliana
SONG Laiqiang, FU Tingdong, MA Caozhi, ZOU Xiaofen 272
Genotype and procedure dependence of Agrobacterium-mediated transformation of Brassica napus
Karin Sonntag 276
Quantitative trait loci influencing heterosis for seedling biomass and seed yield in oilseed rape
Panjisakti Basunanda, Maen Hasan,Tobias Spiller, Rod Snowdon, Wolfgang Friedt 279
Development of allele-specific SNP markers for the new low-linolenic mutant of winter oilseed rape
Katarzyna Mikolajczyk, MirosB
awa Dabert, Wojciech M. Karlowski, Stanislaw Spasibionek, Teresa Cegielska-Taras, Iwona Bartkowiak-Broda 282
Relationship of yielding ability and heterosis effect of winter rapeseed F1 hybrids with genetic distance of parental lines
Alina Liersch,Iwona Bartkowiak-Broda 285
A molecular genetic linkage map in recombinant inbred lines of Brassica napus L. using SSR and SRAP markers
FU Fuyou, LI Jiana 289
Development and characterization of SCAR markers associated with a dominant genic male sterility in rapeseed (Brassica napus. L)
HONG Dengfeng, LIU Jun, YANG Guangsheng, HE Qingbiao 292
Mapping and tagging of agrnomically important genes in Brassica juncea
Vibha Gupta, Akshay K. Pradhan, Naveen C. Bisht, Y.S. Sodhi,N. Arumugam, Arundhati Mukhopadhyay, Deepak Pental 298
Cloning and mapping of a candidate gene for germination and seedling vigour in yellow-seeded oilseed rape
Tung Nguyen, Wolfgang Friedt,Rod Snowdon 301
Molecular evaluation of chemically induced male sterility in Brassica juncea (L.) Czern & Coss.Vandana Singh, S.V.S. Chauhan 304
Inheritance of seed coat color genes of Brassica napus (L.) and tagging the genes using SRAP molecular markers
Mukhlesur Rahman, Peter B.E. McVetty, Genyi Li 310
Development of molecular markers specific to the Ogura fertility restorer gene Rfo in canola (Brassica napus L.)
Xueyi Hu,Mandy Sullivan-Gilbert, Tom Kubik, Jason Danielson, Nathan Hnatiuk, Wesley Marchione, Manju Gupta, Katherine Armstrong, Steven Thompson 314
Identification and mapping of a novel blackleg resistance locus LepR4 in the progenies from Brassica napus × B. rapa subsp.sylvestris 
Fengqun Yu, Derek J. Lydiate, Kristen Hahn, Sarah Kuzmicz, Christine Hammond, S. Roger Rimmer 317
Genetic divergence of rapeseed (Brassica napus L.) seed yield components
Ana Marjanovi
-Jeromela, Radovan Marinkovi
,Dejana Safti
-Pankovi
, Dragana Miladinovi
321
Molecular markers for the seed coat color in Brassica juncea (B3)
YAN Mingli, LIU Zhongsong, GUAN Chunyun, CHEN Sheyuan,YUAN Mouzhi, LIU Xianjun 325
SSR and AFLP fingerprinting of 89 newly bred winter rapeseed cultivars in China
WU Xiaoming, ZHANG Dongxiao, CHEN Biyun,LU Guangyuan, LIU Fenglan 330
Marker-assisted selection of self-incompatible oilseed rape plants.
Jana Zaludova, Bozena Kukolikova, Vladislav Curn 333
Relationship between genetic distance of parent molecular maker and trait performance of hybrid in Brassica napus L.
TANG Zhanglin, LIU Jie, LIU Liezhao, XU Xinfu, WANG Rui, CHEN Li, LI Jiana 336
Analysis of genetic diversity in rapeseed
PU Xiaobin, ZHANG Jinfang, LI Haojie, WANG Maolin, ZHANG Qixing, CAO Yi, LI Yiyi,LI Jiang, LI Xiaoyan, ZHAO Yun, JIANG Liangcai 340
Genetic evolution of Brassica crops revealed with srap markers
WEN Yancheng, WANG Hanzhong, SHEN Jinxiong, LIU Guihua 345
Molecular marker-assisted selection for development of yellow seeded Pol cms restorer lines in Brassica napus L.
LIU Zhiwen,FU Tingdong, TU Jinxing, MA Chaozhi, CHEN Baoyuan, ZHOU Yongming 350

Index to Authors 354